WebNov 12, 2024 · BioID experimental protocol (a) pDEX BASTA (pDB) BioID vector multiple cloning site sequence for cloning in frame with an HA epitope tag (in bold green and underlined).(b) Flowchart of BioID experimental protocol to identify (1) Global proteome of plant organelles/compartments (2) Bait-specific interactions.Abbreviations: M … WebEnzymatic biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site of a newly synthesized enzyme. Biotin protein ligase (BPL) is responsible for this …
A Versatile Lentiviral Delivery Toolkit for Proximity-dependent ...
WebJun 9, 2024 · BioID is based on biotinylation of proteins proximal (~10 nm) to a mutant biotin ligase (BirA*)-fused protein of interest, and the biotinylated proteins can be captured and identified with streptavidin pulldown and LC/MS/MS. Thus, BioID ... vector pDEST-pcDNA5-BirA*-FLAG N-term with LR clonase 2 (Thermo Fisher Scientific) via Gateway system. WebFeb 21, 2024 · The overall process of BioID can be broken down into two stages: (1) generation and characterization of a BioID fusion protein in a mammalian (or other) expression vector for stable expression in a cell line (Basic Protocol 1) and (2) use of that cell line for a large-scale BioID pull-down to identify protein candidates by mass … greenwired solar
A proximity biotinylation-based approach to identify …
WebFeb 24, 2016 · Nup43-Linker-BioID and Nup43-Linker-BioID2 in pcDNA 3.1 vector were subcloned to pBabe.puro using EcoRI and SalI. To generate HA-BioID-LaA and HA-BioID2-LaA, BioID and BioID2 were amplified … WebBioID logo png vector. Download free BioID vector logo and icons in PNG, SVG, AI, EPS, CDR formats. WebPlease acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. A promiscuous biotin ligase fusion … green winter sweaters for men