Do11.10 eg7-ova
WebAug 1, 2000 · To test this hypothesis, we adoptively-transferred DO11.10 OVA-specific T-cell receptor (TCR) transgenic T cells into syngeneic recipients and used a TCR-specific monoclonal antibody to track the in vivo activation of naive CD4 + T lymphocytes following exposure to OVA. The production of OVA-specific antibodies was suppressed in a dose ... WebDec 11, 2024 · EG7-OVA and MC38-OVA cells were subjected to 5 or 10 Gy of radiation (or sham-irradiation) and then labeled with CFSE after 48 h of incubation in complete …
Do11.10 eg7-ova
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WebYou can start by adding a control where Raw cells would be pulsed with the OVA peptide recognized by DO11.10. If it works well, then the absence of detection when the full … WebFeb 28, 2024 · OVA-specific CD4 + DO11.10 cells were isolated as described above and stained with 5 μM CellTrace CTV before incubation with sorted DCs at a ratio of (3:1) and 1 μg/ml Xcl1-OVA, fliC-OVA, or αNIP-OVA for 4 days. As a positive control, cells were incubated with 0.5 μg/ml of the OVA 323-339 peptide. Proliferation of DO11.10 cells were ...
WebApr 1, 2003 · A DO11.10 cell line was generated from DO11.10 TCR-transgenic mice and is specific for ovalbumin (OVA) peptide 323-339 restricted by H2-A d. DAP.3-H2-A k … WebJun 1, 2003 · Fig. 1. Adoptively transferred DO11.10 cells are activated and expand after immunization with OVA ISCOMS. BALB/c mice were adoptively transferred with 3 × 10 6 CD4 + KJ1.26 + cells from DO11.10 mice 2 days prior to immunization with 5 µg OVA in ISCOMS (solid symbols) or no immunization (open symbols). Mice were treated with …
WebNov 20, 2011 · DO11.10 and wild-type mice both developed GBSIII-specific IgM, indicating that lack of IgG production in DO11.10 mice was not due to lack of GBSIII-specific B cells (Supplementary Fig. 8). These ... WebNaive DO11.10 T cells were stimulated with OVA(323 - 329) antigen, and SOCS-3 expression was detected by means of (3)H-TdR. The results showed that after IL-2 pre-stimulation, the proliferation of naive DO11.10 T cells decreased significantly when stimulated with OVA(323 - 329) antigen; SOCS-3 expression of naive DO11.10 T cells …
WebSep 15, 2005 · The DO11.10 cell line is a T-cell hybridoma that recognizes I-A d MHC class II/OVA peptide 323-339 complex (a generous gift from Dr. Louis Falo III). This CD4 + T …
WebMay 11, 2024 · For the tumor rechallenge experiment, cured mice were challenged in the alternate flank subcutaneously with 2 × 10 6 EG7.OVA cells at a 1:1 ratio in RPMI 1640 … spraycoat 1920 testsWebT cells in the DO11.10 TCR transgenic mice (6) are specific for a chicken OVA peptide 323-339/I-A d class II MHC complex (7), and can be detected with the KJ1-26 mAb (8), which is uniquely specific for the DO11.10 clo-notypic TCR. We used this system here, to characterize interactions between peptide–MHC-bearing DC and naive antigen-specific … spray clothingWebOct 9, 2024 · Adoptive Transfer of Ex Vivo Isolated OVA-TCR + CD4 + Cells. On day 0, 5 × 10 6 CFSE-labeled CD4 + cells from DO11.10 were adoptively transferred intravenously (i.v.) into syngeneic BALB/c mice. After 24 hours, mice were treated by a single i.v. injection of PBS (control), unconjugated pOVA or equimolar amounts of PEG-conjugated pOVA as … spray cloroxWeb003303 C.Cg-Tg(DO11.10)10Dlo/J Mice carrying this MHC class II restricted rearranged T cell receptor transgene on a H2d background react to ovalbumin (OVA) peptide antigen. … Please use this form to enter mouse-related technical questions, comments, … MGI Workshops. If you would like to host an MGI workshop at your institution, … Mouse phenome database, MPD...an integrated resource to explore … The Jackson Laboratory is an independent, nonprofit organization focusing on … shenzhen idea light limitedWebTo test this hypothesis, we adoptively-transferred DO11.10 OVA-specific T-cell receptor (TCR) transgenic T cells into syngeneic recipients and used a TCR-specific monoclonal … shenzhen idd codeWebAug 1, 1999 · Abstract. The majority of clonotypic CD4 + T cells in the intestinal lamina propria of DO11.10 TCR transgenic mice have an activated/memory phenotype and produce effector cytokines despite the absence of prior exposure to ovalbumin (OVA), the transgene-specific antigen. A small number of splenic T cells have a similar phenotype. Clonotypic … sprayco all purpose sprayer adjustmentsWebFeb 1, 2004 · The triggering Ag for inflammatory bowel disease and animal models of colitis is not known, but may include gut flora. Feeding OVA to DO11.10 mice with OVA-specific transgenic (Tg) TCR generates Ag-specific immunoregulatory CD4(+) T cells (Treg) cells. We examined the ability of oral Ag-induced Treg cells to suppress T cell-mediated colitis … shenzhen igear technology inc