WebFixable Viability Stain 510 labeling of cells. 1. Prepare cells for flow cytometry staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1x10^6 cells/ml in sodium azide- and protein-free 1X DPBS. 4. WebFixable Viability Stain 780 labeling of cells. 1. Prepare cells for flow cytometric staining using sodium azide-free buffers. 2. Wash cells one …

DRAQ5™ - BD Biosciences

WebThaw vial of dye. 2. Dilute LIVE/DEAD fixable dead cell stain by adding 50 µL DMSO to vial. 3. Add 1 mL of cells to a flow cytometer tube in protein-free buffer. 4. Add 1 µL of diluted stain to cells. 5. Mix cells and stain. WebThe LIVE/DEAD Fixable Red (615) Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ... litezpeed wholesale skateboard distribution

eBioscience™ Fixable Viability D ye eFluor™ 780

Web7. [Optional] Stain cells for surface markers. Refer to "Staining Cell Surface Targets, Protocol A" found in our Best Protocols. 8. Analyze samples by flow cytometry. Protocol … WebNov 19, 2024 · Pro tip: make sure to read the manufacturer’s protocol for your chosen viability stain. Non-fixable dyes like PI, DAPI, and the Sytox dyes should be stained after the antibody stain step and do not need to … WebAfter being stained for 10 min with Fixable Viability Stain 440UV (BD Biosciences, NJ, USA), fixed for 50 min with Transcription Factor Buffer (562574, BD Biosciences, NJ, USA) at 4°C, and washed by 1X Perm/Wash buffer (BD Biosciences, NJ, USA), the stained cells were incubated for 50 min with Alexa Flour 647 anti-mouse FOXP3 (126408 ... litfad bench