Hoechst 33342 molecular probes

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August undefined, 2024

NettetHoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content. NettetThermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.Features of Hoechst 33342 Fluorescent Stain:

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Nettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … NettetBisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several related chemical compounds are used for similar purposes and are … hair 11306

Hoechst 33342 - Handbook of Fluorescent Dyes and Probes

Nettet2. jan. 2009 · Spectral data for Hoechst 33342 and DCV are courtesy of Molecular Probes Invitrogen. Abbreviation: DCV, DyeCycle Violet. For the many laboratories that do not have access to a UV-equipped flow cytometer, therefore, the problem of performing Hoechst SP analysis remains. NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has … hair 1122

Invitrogen™ Hoechst 33342, Trihydrochloride, Trihydrate - 10 …

NettetAfter removing MitoTracker Red CMXRos, cells were treated with 200 nM MitoTracker Green FM (Molecular Probes) and incubated at 37 °C for 30 min. After removing MitoTracker Green FM, cells were treated with Hoechst 33342 (Dojindo) and incubated at 37 °C for 30 min. The fluorescence intensity of MitoTracker Red CMXRos, ... hair 10x growthNettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell-permeability than H 33258. Cells can integrate BrdU in newly synthesized DNA as a substitute for thymidine. brand staffing

"NettetHoechst 33342 was also used to stain the cellular nuclei. As shown in Figure 2, in Ir-treated group, ... Smith RA, Hartley RC, Murphy MP. Mitochondria-targeted small molecule therapeutics and probes. Antioxid Redox Signal. 2011;15(12):3021–3038. 33. " - Hoechst 33342 molecular probes

Hoechst 33342 molecular probes

Photothermal exposure of polydopamine-coated branched Au–Ag ...

NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … Nettet(B) Co-staining of viscosity-sensitive probes (DSP-16 and DSP-18), Dil (a commercial plasma membrane probe) with Hoechst 33342 (a commercial nuclei probe) …

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NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570). Apoptosis, Cell Analysis, Cell Cycle, Cell ... NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell …

Nettet20. feb. 2015 · This chapter lists the CAS registry number, chemical structure, chemical abstract (CA) index name, other chemical names, Merck Index number, chemical/dye … NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed …

Nettet15. mar. 2013 · Amplex Red cholesterol assay kit was from Molecular Probes (Eugene, OR). All other chemicals and solvents used were of the highest available purity. Water was purified through a Millipore ... DNA bound Hoechst 33342 was excited with 351 nm and fluorescence was collected using 450/65 nm bandpass filter. In order to ... NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the …

NettetbisBenzimide H 33342 trihydrochloride Synonym(s) : 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1 H -benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, …

NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from ... Molecular Probes, Inc. Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly hair 10x growth oil reviewsNettet(Molecular Probes: C-1430). Hoechst 33342, a bisbenzimide dye, is a cell membrane permeant, minor groove-binding DNA stain that fluoresce bright blue upon binding to DNA. The dye is quite soluble in water and relatively non-toxic (Molecular Probes: H-1399, H-3570). Hoechst 33342 stains both, live and dead cells. Material and Methods Cell Culture hair 11 fordingbridgeNettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … brands supporting mental healthNettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue™ Live ReadyProbes™ Reagent we … hair 119NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570). brands taiwanNettetBiotium offers both Hoechst 33342 and Hoechst 33258, ... Note: DAPI or Hoechst can be combined with other fluorescent probes. ... Molecular formula: C 27 H 31 Cl 3 N 6 O; CAS number: 23491-52-3; Recommended staining concentration: 1 ug/mL; Hoechst 33342, trihydrochloride trihydrate. hair 10 year oldshttp://www.cogershop.com/hoechst-33342.htm brands taking stands on social issues